Publicacións

O CITEEC lidera a investigación en enxeñaría civil e edificación, enfocándose en solucións innovadoras para infraestruturas sostibles e materiais avanzados.

Home > Publicacións

publicacións

Investigación de vangarda para o desenvolvemento sostible

O CITEEC realiza investigación avanzada en enxeñaría civil, materiais, sustentabilidade e infraestruturas. A través de proxectos multidisciplinarios e colaboracións internacionais, xéranse publicacións científicas que abordan retos crave do sector. Nesta sección preséntanse as principais publicacións e resultados obtidos polos nosos grupos de investigación.

Accede a unha ampla variedade de publicacións científicas filtradas por temática, autor, ano e máis. Utiliza os filtros dispoñibles para atopar investigacións relevantes e obter detalles sobre cada unha das nosas contribucións ao avance do coñecemento.

Investigación de vangarda para o desenvolvemento sostible

Show all

2026

Kubista, Mikael; Forootan, Amin; Pfaffl, Michael W.; Bustin, Stephen A.; Andrade-Garda, José Manuel; Sjöback, Robert; Sjögreen, Björn; Ståhlberg, Anders

Understanding the qPCR Standard Curve: From Assay Validation to Absolute Quantification and Variance PCR Journal Article

In: International Journal of Molecular Sciences, vol. 27, no. 6, pp. 2904, 2026, ISSN: 1422-0067.

Abstract | Links | BibTeX | Etiquetas: MIQE, molecular diagnostics, PCR, qPCR, real-time PCR, RT-PCR, standard curve, variation PCR, varPCR

@article{kubista_understanding_2026,

title = {Understanding the qPCR Standard Curve: From Assay Validation to Absolute Quantification and Variance PCR},

author = {Mikael Kubista and Amin Forootan and Michael W. Pfaffl and Stephen A. Bustin and José Manuel Andrade-Garda and Robert Sjöback and Björn Sjögreen and Anders Ståhlberg},

url = {https://www.mdpi.com/1422-0067/27/6/2904},

doi = {10.3390/ijms27062904},

issn = {1422-0067},

year  = {2026},

date = {2026-01-01},

urldate = {2026-08-18},

journal = {International Journal of Molecular Sciences},

volume = {27},

number = {6},

pages = {2904},

publisher = {Multidisciplinary Digital Publishing Institute},

abstract = {The quantitative polymerase chain reaction (PCR) standard curve is the central analytical tool for validating qPCR assays and can also be used to estimate target concentrations in test samples. This review explains how qPCR standard curves are constructed, validated, and analyzed for different purposes. We first examine an idealized standard curve generated using an exceptionally high number of replicates, far exceeding typical routine use. This approach clearly illustrates fundamental qPCR characteristics and provides an educational framework for defining and estimating PCR efficiency, limit of detection, and limit of quantification. Furthermore, we demonstrate that, in theory, variation in threshold crossing points across replicates can be used to estimate the number of target molecules in a sample. This method, which we term variance PCR, could complement digital PCR and potentially extend the dynamic range of absolute quantification. We also analyze a representative standard curve as typically processed in routine qPCR workflows. This includes validating its dynamic range, assessing the impact of outliers, estimating PCR efficiency and precision, and finally applying the curve to determine the concentration of test samples.},

keywords = {MIQE, molecular diagnostics, PCR, qPCR, real-time PCR, RT-PCR, standard curve, variation PCR, varPCR},

pubstate = {published},

tppubtype = {article}

}